HOME NEWS TECHNOLOGY SERVICES ABOUT US CONTACT US   Kit Services | In House Services | Cis Platform | Trans Platform | Facilities     Factorial30™ reagent kit related services We generate custom Factorial30™ systems that fit particular cell type. About custom Factorial30™ systems please inquire here. We can process CE samples for our Factorial™ kit clients who don’t have this capability. CE samples submission form with instructions can be downloaded here. Back to the top In House FACTORIAL™ Services Based on its proprietary Factorial™ technology, Attagene has developed two distinct cell-based assays useful for evaluation of mode of action and toxicity of chemical compounds and drug candidates. Cis-Factorial™ assay reports effects of compounds on activity of more than 50 transcription factors in toxicologically relevant human cell lines. Trans-Factorial™ assay evaluates agonistic/antagonistic properties of compounds across 25 human hormone nuclear receptors. Both assays have been advanced into high-throughput technological platforms and were selected by the US EPA ToxCast project that seeks to identify predictive bioactivity signatures of environmental toxicants. The assays are now available to large chemical and drug-development customers thru our In House Factorial™ services. For an illustration of how Cis-Factorial™ assay helps assessing mode of action and specificity of drugs, click here . Back to the top Cis-Factorial™ Platform: Provides assessment of compounds across broad spectrum of TF activities. Including: NF-kB – inflammation, autoimmunity p53 – genotoxic stress, cancer HIF1a – cardiovascular problems NF-E2 – oxidative stress, ageing and many others *Currently 50 validated TFs and growing….. Click here for complete list cis-FactorialTM endpoints. Back to the top Trans-Factorial™ Platform: Provides assessment of agonistic/antagonistic effects of compounds on multiple NRs. Including: PXR – drug metabolism ERa, THRa – endocrine disruption PPARg – obesity LXRb– liver toxicity and many others *Currently 24 NRs and growing….. Click here for complete list trans-FactorialTM endpoints. Back to the top Our in house custom services are performed @ our GLP validated facilities in RTP, NC, USA. The facilities include: Factorial™ Detection Unit performs all steps that required by the Factorial™ detection protocol, including total RNA isolation, reverse transcription, PCR amplification, fluorescent labeling, restriction digest, sample clean-up, capillary electrophoresis, primary data analysis and storage of the data. Tissue Culture Facility The Facility performs cell-based steps of the Factorial™ assay including cell storage, propagation, cell plating for screening experiments, transfection and exposure to compounds. The facility is in compliance with bio safety level 2 and occupies two rooms. Customer samples handling and storage facility The facility performs signing in, aliquoting, reformatting and storage of the samples received from the customers. The samples may include stabilized cell lyzates, RNA preps and chemical compounds. Facility is built to be compliant with OSHA and NCDENR regulations. Back to the top        ©Attagene,Inc; All rights reserved. LEGAL SITE MAP CUSTOMER SERVICE